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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
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Immunology and Infection
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JoVE Journal Immunology and Infection
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

DOI:
10.3791/52564-v

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10:31 min

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April 08, 2016

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, , , ,
1Laboratory of Medical Immunology, Department of Laboratory Medicine,Radboud University Medical Centre, 2Department of Dermatology,Radboud University Medical Centre

Chapters

  • 00:05Title
  • 01:19Preparation of Single Cell Suspensions from Human Skin
  • 04:54Polyclonal Activation of Skin-resident T Cells and Flow Cytometry Analysis
  • 08:04Results: Phenotypic Analysis of Skin-derived Lymphocytes
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

Tags

Keywords: Lymphocyte IsolationSkin BiopsyT CellsFlow CytometryEx Vivo Cell CultureSingle Cell Suspension

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