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JoVE Journal
Immunology and Infection

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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
 

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Article doi: 10.3791/52564
April 8th, 2016

Summary April 8th, 2016

We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

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