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JoVE Journal
Immunology and Infection

This content is Open Access.

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
 

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Article doi: 10.3791/52610
May 4th, 2015

Summary May 4th, 2015

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

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