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Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

DOI:
10.3791/52610-v

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11:19 min

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May 04, 2015

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, , , , , , , , , , ,
1Department of Microbiology,University of Washington, 2Departments of Medicine and Laboratory Medicine,University of Washington, 3U.S Military HIV Research Program,Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

Chapters

  • 00:05Title
  • 01:23Growth Competition
  • 05:00Determination of Viral Ratios
  • 09:16Results: HIV Replication Fitness Determined by Pairwise Growth Competition Assays
  • 10:54Conclusion

Summary

Automatic Translation

Automatic Translation

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

Tags

Keywords: HIV-1Viral Replication FitnessIn Vitro Fitness AssayGrowth Competition AssayMultiplicity Of Infection (MOI)Viral DetectionRelative Fitness Calculation

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