Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Siriphan Manocheewa; Erinn C. Lanxon-Cookson; Yi Liu; J. Victor Swain; Jan McClure; Ushnal Rao; Brandon Maust; Wenjie Deng; Justine E. Sunshine; Moon Kim; Morgane Rolland; James I. Mullins

doi:10.3791/52610

Journal

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Immunology and Infection

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Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

11,087 Views

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11:19 min

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May 04, 2015

DOI:

10.3791/52610-v

DOI:

10.3791/52610-v

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11:19 min

May 04, 2015

•

10 Views

Siriphan Manocheewa¹, Erinn C. Lanxon-Cookson¹, Yi Liu¹, J. Victor Swain¹, Jan McClure¹, Ushnal Rao¹, Brandon Maust¹, Wenjie Deng¹, Justine E. Sunshine¹, Moon Kim¹, Morgane Rolland^3,4, James I. Mullins^1,2

¹Department of Microbiology,University of Washington, ²Departments of Medicine and Laboratory Medicine,University of Washington, ³U.S Military HIV Research Program,Walter Reed Army Institute of Research, ⁴Henry M. Jackson Foundation

Summary

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.