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A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity
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JoVE Journal Neuroscience
A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity

A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity

DOI:
10.3791/52642-v

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10:05 min

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May 07, 2017

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, , , ,
1Canadian Center for Behavioural Neuroscience,University of Lethbridge

Chapters

  • 00:05Title
  • 01:01Surgery Preparation
  • 02:28Removing the Skin and Retracting the Muscle from the Skull
  • 04:35Craniotomy
  • 06:36Dura Removal and Cranial Window Preparation
  • 08:15Results: Unique and Consensus Activation Patterns during Multiple Forms of Sensory Stimulation
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a method for creating a large unilateral craniotomy over the temporal and parietal regions of the mouse cerebral cortex. This is especially useful for real time imaging over an expansive area of a cortical hemisphere.

Tags

Keywords: Large Lateral CraniotomyMesoscale Wide-field Optical ImagingBrain ActivityNeural DynamicsBrain Circuit DevelopmentOptogeneticsAlzheimer’s DiseaseStrokeAnesthetized MouseThermo-regulating Heating PadDexamethasoneLidocaineDissecting MicroscopeTemporal Muscle

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