Mouse Brain Surgery
Mouse Brain Surgery - Related Videos
09:25
A Craniotomy Surgery Procedure for Chronic Brain Imaging
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Cited by 182 •
2008
This video and protocol demonstrate how to implant a glass-covered cranial window in rodents. These preparations can be used for chronic in vivo two-photon imaging of the neocortex over time scales of months. It may also be used for other types of imaging, including optical intrinsic signal imaging.
12:45
Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
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Cited by 22 •
2012
Targeted ablation of specific brain region(s) by infusion of an excitotoxin using stereotaxic coordinates is described. This technique could also be adapted for infusion of other chemicals into the rat brain.
09:44
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
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Cited by 19 •
2018
We describe a protocol of stereotaxic surgery with a homemade head-fixed device for microinjecting reagents into the striatum of neonatal mouse brains. This technique allows genetic manipulation in neuronal cells of specific regions of neonatal mouse brains.
08:28
Rodent Stereotaxic Surgery
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2023
Stereotaxic (or stereotactic) surgery is a method used to manipulate the brain of living animals. This technique allows researchers to accurately target deep structures within the brain through the use of a stereotaxic atlas, which provides the 3D coordinates of each area with respect to anatomical landmarks on the skull. After the skull is exposed, anesthetized animals are mounted on a specialized instrument known as a stereotaxic frame, which enables the precise placement of experimental...
08:32
Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury
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Cited by 12 •
2017
We performed unilateral carotid artery occlusion on postnatal day 7-10 CD-1 mouse pups to create a neonatal hypoxic-ischemic (HI) model and investigated the effects of HI brain injury. We studied neurobehavioral functions in these mice compared to non-operated normal mice.
FAQs
Stereotaxic positioning converts selected brain coordinates into a reproducible instrument path. The equipment stabilizes the mouse’s head so researchers can guide instruments to the intended location rather than relying on unrestricted hand placement. This precision supports targeted craniotomies, substance delivery, or device placement and improves comparability among experiments and animals.
A craniotomy creates access through the skull at the selected site. Once access is established, the researcher can perform the planned manipulation, introduce a substance, or place a recording device. The opening therefore links the coordinate-based setup to the experiment’s specific neural intervention, while surrounding surgical controls help preserve reliable observations.
Anesthesia, aseptic technique, and temperature control serve different but complementary purposes. Anesthesia supports the surgical procedure, aseptic practice helps limit contamination, and temperature control helps maintain stable surgical conditions. Together with careful postoperative recovery, these measures protect animal welfare and reduce condition-related variation that could complicate interpretation of neural or behavioral data.
The same general surgical framework can support either intervention or observation, depending on the intended study. Researchers may deliver substances to examine drug or gene effects, manipulate a selected brain region, or place recording devices to monitor activity. This flexibility allows experiments to connect targeted neural changes with later biological or behavioral outcomes.
These procedures support studies of neural circuits, behavior, disease, neurodegeneration, and brain injury. By accessing defined brain locations, researchers can examine how targeted manipulations or recordings relate to broader biological responses. The approach is especially useful when investigators need to connect activity or treatment at the neural level with changes in behavior or disease-related processes.
Mouse brain surgery provides a way to link cellular mechanisms with outcomes observed in a living organism. Researchers can investigate the effects of genes or drugs, examine neural-circuit function, and study conditions such as neurodegeneration or brain injury. These connections help place cellular and neural events within broader biological processes involving behavior and disease.