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Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines

Article DOI: 10.3791/52671
March 22nd, 2015 •
*1, *1, 1, 1, 1
1Department of Surgery and Cancer, Imperial College London
* These authors contributed equally

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March 22nd, 2015

Floating mammosphere assays can investigate the subset of stem-like breast cancer cells that survive in suspension conditions and show enhanced tumorigenesis when implanted into mice. This protocol provides a convenient in vitro measure of sphere-forming ability, a proxy for in vivo tumorigenesis, while facilitating analysis of the stem-associated transcriptional landscape.

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