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Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
 

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines

Article DOI: 10.3791/52671 10:51 min
March 22nd, 2015

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Summary March 22nd, 2015

Floating mammosphere assays can investigate the subset of stem-like breast cancer cells that survive in suspension conditions and show enhanced tumorigenesis when implanted into mice. This protocol provides a convenient in vitro measure of sphere-forming ability, a proxy for in vivo tumorigenesis, while facilitating analysis of the stem-associated transcriptional landscape.

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