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Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons
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Neuroscience
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JoVE Journal Neuroscience
Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

DOI:
10.3791/52730-v

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12:19 min

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June 24, 2015

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, ,
1Department of Neurobiology and Behavior,Stony Brook University, 2Department of Pharmacological Science,Stony Brook University

Chapters

  • 00:05Title
  • 02:26vHipp-nAcc Synaptic Co-cultures
  • 06:13FM1-43 Based Vesicular Fusion
  • 08:12Calcium Imaging
  • 09:35Results: Synaptic Co-culture of vHipp with nAcc allows Examination of Pre-synaptic Localization of nAChRs
  • 10:57Conclusion

Summary

Automatic Translation

Automatic Translation

We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.

Tags

Keywords: Live ImagingNicotineCalcium SignalingNeurotransmitter ReleaseVentral HippocampusAxonsNicotinic Acetylcholine ReceptorsSynaptic PlasticityGene-chimeric PreparationVentral Hippocampus-accumbens CircuitGlutamatergic SynapsesPre-synaptic Nicotinic SignalingElectrophysiologyLive Cell Imaging

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