8,954 Views
•
12:19 min
•
June 24, 2015
DOI:
Summary
Automatically generated
We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.
Explore More Videos
Related Videos
08:38
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
Related Videos
15479 Views
08:47
Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
Related Videos
13410 Views
09:34
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
Related Videos
15691 Views
10:48
Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine
Related Videos
9191 Views
12:16
Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice
Related Videos
7160 Views
09:07
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia
Related Videos
2633 Views
01:24
Co-culturing of Ventral Hippocampal and Nucleus Accumbens
Related Videos
55 Views
01:29
Imaging Nicotine-Induced Calcium Signaling along Ventral Hippocampal Axons in Synaptic Co-Cultures
Related Videos
35 Views
12:19
Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons
Related Videos
9.0K Views
01:29
Imaging Nicotine-Induced Calcium Signaling along Ventral Hippocampal Axons in Synaptic Co-Cultures
Related Videos
35 Views
Read Article
Cite this Article
Zhong, C., Talmage, D. A., Role, L. W. Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons. J. Vis. Exp. (100), e52730, doi:10.3791/52730 (2015).
Copy