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Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus
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JoVE Journal Neuroscience
Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

DOI:
10.3791/52805-v

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08:16 min

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June 17, 2015

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, , , ,
1Department of Pathology and Cell Biology,Columbia University Medical Center, 2The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain,Columbia University Medical Center, 3Department of Neurology,Columbia University Medical Center

Chapters

  • 00:05Title
  • 01:18Animal Preparation
  • 02:16Surgery and Infusion
  • 05:40Animal Removal and Postoperative Care
  • 06:23Results: Oligomeric Aβ1-42 Induces Neuron Death in the Dentate Gyrus and the Basal Forebrain
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for direct stereotaxic brain infusion of amyloid-beta. This methodology provides an alternative in vivo mouse model to address the short-term effects of amyloid-beta on brain neurons.

Tags

Keywords: Alzheimer’s DiseaseAmyloid-betaOligomeric Amyloid-betaStereotaxic InfusionHippocampusDentate GyrusBasal ForebrainNeuron LossMouse Model

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