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In vitro e in vivo modelo para estudiar la adhesión bacteriana a la pared del recipiente en condiciones de flujo
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
DOI:

10:24 min

June 11, 2015

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Chapters

  • 00:05Title
  • 01:40S. aureus Preparation for In Vitro Perfusion and In Vivo Experiments
  • 03:00In Vitro Perfusion Experiments
  • 05:53In Vivo Mesentric Perfusion Model
  • 08:08Results: von Willebrand Factor is Essential for Shear Mediated Adhesion of S. aureus to Endothelial Cells and to the Subendothelial Matrix
  • 09:38Conclusion

Summary

Automatic Translation

To study the interaction of bacteria with the blood vessels under shear stress, a flow chamber and an in vivo mesenteric intravital microscopy model are described that allow to dissect the bacterial and host factors contributing to vascular adhesion.

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