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Implementering Patch Clamp og Live Fluorescensmikroskopi til Monitor funksjonelle egenskaper nyisolerte PKD Epitel
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JoVE Journal Biology
Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementering Patch Clamp og Live Fluorescensmikroskopi til Monitor funksjonelle egenskaper nyisolerte PKD Epitel

DOI:
10.3791/53035-v

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08:46 min

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September 01, 2015

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, , , , ,
1Department of Physiology,Medical College of Wisconsin, 2Department of Integrative Biology & Pharmacology,University of Texas Health Science Center at Houston

Chapters

  • 00:05Title
  • 02:14Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
  • 05:02Single Channel Patch-clamp Electrophysiology
  • 05:32Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
  • 06:55Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

Ionekanaler uttrykt i nyretubuli epitel spille en betydelig rolle i patologi polycystisk nyresykdom. Her beskriver vi eksperimentelle protokollene som brukes til å utføre patch-clamp analyse og intracellulær kalsium nivå målinger ved cystisk epitel fersk isolert fra gnager nyrer.

Tags

Patch ClampLive Fluorescence MicroscopyPolycystic Kidney DiseasePKDARPKDIon ChannelsCalcium SignalingEpithelial MonolayerEx Vivo AnalysisPCK RatSprague Dawley RatNative SettingEpithelial IsolationImmunostainingPrimary CultureBiochemical Assays

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