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Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
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Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

DOI:
10.3791/53069-v

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08:47 min

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March 30, 2016

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, , , , ,
1Department of General-, Visceral- and Transplantation Surgery,Charité University Medicine Berlin, 2Brandenburg Center for Regenerative Therapies,Charité University Medicine Berlin

Chapters

  • 00:05Title
  • 00:33Perfusion and Digestion of Liver Tissue Sample
  • 02:36Isolation of Hepatocytes
  • 03:56Isolation of Non-parenchymal Liver Cells
  • 05:16Separation of Kupffer Cells, Endothelial Cells, and Stellate Cells
  • 07:07Results: Morphology of Human Parenchymal and Non-parenchymal Liver Cells in Suspension and After Adherence
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

A technique to isolate human hepatocytes and non-parenchymal liver cells from the same donor is described. The different liver cell types build the basis for functional liver models and tissue engineering. This new method aims to isolate liver cells in a high yield and viability.

Tags

Keywords: Primary Human HepatocytesNon-parenchymal Liver CellsIsolationPerfusionCollagenase DigestionBuchner FunnelPeristaltic PumpIn Vitro Co-culture

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