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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
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JoVE Journal Neuroscience
Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

DOI:
10.3791/53369-v

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10:31 min

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September 29, 2017

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, , ,
1University of Michigan Medical School, 2Department of Neurology,University of Michigan, 3Department of Internal Medicine,University of Michigan

Chapters

  • 00:05Title
  • 01:02Fluorescence Immunohistochemistry: Day 1
  • 02:27Fluorescence Immunohistochemistry: Day 2
  • 03:21Fluorescence Immunohistochemistry: Day 3
  • 04:22Confocal Imaging
  • 06:00Image Processing
  • 07:03Isolation of Nerve-specific Mitochondrial Signal
  • 08:41Results: Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol uses three-dimensional (3D) imaging and analysis techniques to visualize and quantify nerve-specific mitochondria. The techniques are applicable to other situations where one fluorescent signal is used to isolate a subset of data from another fluorescent signal.

Tags

Keywords: 3D ImagingMitochondriaIntraepidermal Nerve FibersFluorescence ImmunohistochemistryPGP9.5PDHSecondary AntibodiesSignal EnhancementEpidermal Nerve FibersNeurological Complications

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