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Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Bioengineering
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JoVE Journal Bioengineering
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

DOI:
10.3791/53447-v

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11:51 min

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March 01, 2016

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, , , , ,
1Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, 2The Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, 3Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine,University of Hong Kong

Chapters

  • 00:05Title
  • 00:58Cell Culture and Sorting of Differentiated Cardiomyocytes
  • 04:50Collection of Reaggregated Cardiac Cells
  • 06:32Generation of Human Engineered Cardiac Tissues
  • 09:45Results: Contractile Force Measurement Using Human Engineered Cardiac Tissues
  • 11:05Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.

Tags

Cardiac Cell TherapyCardiac Contractile FunctionCardiac TherapeuticsHuman Embryonic Stem Cell-derived CardiomyocytesFACSSIRP Alpha-PE/Cy7CD90-FITC

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