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Journal / Immunology and Infection / Gene Expression Profiling of Infecting Microbes…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
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Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Article DOI: 10.3791/53460-v • 09:13 min • January 13th, 2016
January 13th, 2016 •
1, 2, 2, 1
1Department of Biological Sciences, Carnegie Mellon University, 2Infectious Diseases, Harbor-UCLA Medical Center

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Summary
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We developed a protocol that is fast, sensitive and reproducible for pathogen gene expression profiling during an infection.

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Gene Expression Profiling Infecting Microbes Digital Bar-coding Platform Pathogen Gene Expression In Vivo Infectious Environment Host Damage Infectious Disease Tissue Types Time Points Sensitivity Reproducibility Tiny Amount Of Tissues Reagents Preparation 2-Mercaptoethanol Buffer RLT Phenol-chloroform Isoamyl Alcohol Solution M-type Homogenization Tubes Zirconia Beads Instruments Tissue Dissociator Bead Beater Tabletop Centrifuge Photometer Kidneys Isolation C.albicans-infected Mice
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