Journal
/
Biology
/
Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

DOI:
10.3791/53469-v

•

10:41 min

•

December 16, 2015

•

, , , ,
1Buchmann Institute for Molecular Life Sciences and Institute of Biochemistry II, School of Medicine,Goethe University

Chapters

  • 00:05Title
  • 00:523D Cell Shape Model Reconstruction with IMOD
  • 03:08Fluorescently-labeled Structure Tracking with Endrov
  • 05:33Cortical Actomyosin Flow Analysis with PIVIab
  • 08:04Results: Representative C. elegans Developmental Tracking Analyses
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.

Tags

Keywords: C. ElegansDevelopmental ProcessesTime-lapse MicroscopyTrackingQuantificationOpen-source ToolsIMODEndrovPIVlabCell TrackingLineage TracingVesicle Flow

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53469?article_type=v

Using Micro-Electro-Mechanical Systems (MEMS) to Develop Diagnostic Tools
check_url/53469?article_type=v

Generation of Stable Transgenic C. elegans Using Microinjection
check_url/53469?article_type=v

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
check_url/53469?article_type=v

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
check_url/53469?article_type=v

Antibody Staining in C. Elegans Using "Freeze-Cracking"
check_url/53469?article_type=v

Methods for Skin Wounding and Assays for Wound Responses in C. elegans
check_url/53469?article_type=v

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
check_url/53469?article_type=v

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
check_url/53469?article_type=v

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
check_url/53469?article_type=v

Measuring RAN Peptide Toxicity in C. elegans

Read Article