Vesicle Flow Tracking
Vesicle Flow Tracking - Related Videos
07:29
Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry
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2023
Here, we describe a straightforward protocol that enables in vitro assessment of the abundance of fluorescently labeled microRNAs to study the dynamics of microRNA packaging and export into extracellular vesicles (EVs).
09:39
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
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Cited by 31 •
2015
Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.
06:28
Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
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Cited by 9 •
2017
Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.
02:43
Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions
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2025
In this video, we demonstrate the interaction between proteins and artificial phospholipid vesicles using quantitative flow cytometry. The binding of fluorescently-labeled proteins to fluorescently-labeled phospholipid vesicles increases the mean fluorescence intensity, and this increase is detected by a flow cytometer.
08:11
Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
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Cited by 19 •
2013
Cilia-generated fluid flow in Kupffer’s Vesicle (KV) controls left-right patterning of the zebrafish embryo. Here, we describe a technique to modulate gene function specifically in KV cells. In addition, we show how to deliver fluorescent beads into KV to visualize fluid flow.
FAQs
Trajectory direction, velocity, and pauses provide complementary information about intracellular transport. Direction shows whether movement follows a particular route, velocity describes how rapidly a vesicle travels, and pauses identify interruptions in delivery. Examining these features together helps researchers evaluate how vesicles move through the cell rather than relying only on their locations at individual time points.
Motor proteins and cytoskeletal structures help organize vesicle movement along intracellular tracks. Their influence can appear as differences in travel direction, velocity, or the frequency and duration of pauses. Vesicle flow tracking therefore connects observable motion with the cellular machinery that supports cargo delivery, allowing researchers to assess how transport is regulated within cells.
Changes in vesicle motion under different cellular conditions can show how transport is regulated or disrupted. Altered trajectories, velocities, or pauses may provide evidence that cargo delivery between organelles or to the cell surface has changed. These measurements help link cellular state to transport behavior without depending solely on static structural observations.
A typical workflow labels the vesicles of interest, records their movement using time-lapse microscopy, and follows individual trajectories across successive images. Researchers then analyze direction, velocity, and pauses to quantify transport behavior. This sequence converts changing vesicle positions into measurements that can be compared across cellular conditions or experimental settings.
The approach is useful when researchers need quantitative information about dynamic cargo transport. It supports studies of secretion, endocytosis, and neuronal transport, where vesicles must move through organized intracellular pathways. By measuring motion over time, investigators can examine delivery processes that may be difficult to evaluate from static images alone.
Vesicle flow tracking can provide quantitative evidence of transport defects associated with disease-related trafficking problems. Comparing vesicle trajectories, velocities, and pauses can reveal altered movement patterns and help identify changes in intracellular delivery. In biology research, these observations connect cellular transport behavior with defects affecting organelles or delivery to the cell surface.