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Chemistry

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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
 

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

Article DOI: 10.3791/53575
March 14th, 2016

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Summary March 14th, 2016

The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.

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