Journal
/
Immunology and Infection
/
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 3. Aerobiology
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 3. Aerobiology

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 3. Aerobiology

DOI:
10.3791/53602-v

•

11:13 min

•

October 03, 2016

•

, , , , , , , , , , ,
1Integrated Research Facility at Frederick, National Institute of Allergy and Infectious Diseases (NIAID),National Institutes of Health (NIH)

Chapters

  • 00:05Title
  • 00:52Nonhuman Primate Transport and Handling: Animal Biosafety Level 4 Suit Laboratory
  • 02:08Aerobiology: ABSL-4 Cabinet Laboratory
  • 08:33Results: Biosafety Practices for Aerobiology within an ABSL-4 Suit and ABSL-4 Cabinet Laboratory
  • 10:11Conclusion

Summary

Automatic Translation

Automatic Translation

As high-consequence pathogens can potentially infect subjects through airborne particles, aerobiology has been increasingly applied in pathogenesis research and medical countermeasure development. We present a detailed visual demonstration of aerobiology procedures during an aerosol challenge in nonhuman primates in an animal biosafety level 4 maximum containment environment.

Tags

Keywords: BiosafetyAerobiologyAerosolClass III Biosafety CabinetNegative-pressureRapid Transfer PortRTPNonhuman PrimateNHPAnesthesiaSharpsTransport CartDecontaminationO-ringsDunk TankDisinfectant

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53602?article_type=v

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
check_url/53602?article_type=v

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
check_url/53602?article_type=v

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice
check_url/53602?article_type=v

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
check_url/53602?article_type=v

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
check_url/53602?article_type=v

Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
check_url/53602?article_type=v

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
check_url/53602?article_type=v

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures
check_url/53602?article_type=v

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices
check_url/53602?article_type=v

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures

Read Article