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Chapters
- 00:05Title
- 00:42Hippocampal Dissection and Plating
- 03:32Astrocyte Culture
- 05:34Oxygen and Glucose Deprivation and Reoxygenation (OGD/REOX)
- 06:21Results: Increased Protein Expression of HIF1α and Astrocytic Markers Following OGD/REOX
- 07:46Conclusion
Astrocytes are one of the most important key players in the central nervous system (CNS). Here, we are reporting a practical method of sexed hippocampal astrocyte culture protocol in order to study the mechanisms underlying the astrocyte function in male and female neonate pups after in-vitro ischemia.
Tags
Sex DifferencesMouse Hippocampal AstrocytesIn-vitro IschemiaEnriched CulturesOxygen And Glucose DeprivationReoxygenationHypoxic IschemiaMechanistic QuestionsTranslational QuestionsDeveloping Male And Female BrainsBrain DissectionNewborn Mouse PupPosterior To Anterior Midline IncisionCranium RemovalBrainstem RemovalDissecting DishHemispheres SeparationCerebral Hemispheres PeelingHippocampus RemovalMeninges And Fibroblasts Cleaning
