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Neuroscience

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Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
 

Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons

Article DOI: 10.3791/53697
February 10th, 2016

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Summary February 10th, 2016

Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.

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