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Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

DOI:
10.3791/53853-v

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09:49 min

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April 15, 2016

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, , , , ,
1Department of Pharmacology,Medical University of Innsbruck, 2Hertie Institute for Clinical Brain Research and Centre for Integrative Neuroscience,University of Tübingen

Chapters

  • 00:05Title
  • 01:30Specimen Preparation
  • 02:22High Pressure Freezing
  • 03:49Preparation of the Electron Beam Gun
  • 04:42Freeze-fracture and Replication
  • 06:26Immunolabeling and Analysis
  • 07:18Results: AMPA-Rs and NMDA-Rs Immunolabeling at Identified PIN/MGn-ITC Synapses
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

This article illustrates how the expression of neurotransmitter receptors can be quantified and the pattern analyzed at synapses with identified pre and postsynaptic elements using a combination of viral transduction of optogenetic tools and the freeze-fracture replica immunolabeling technique.

Tags

Keywords: OptogeneticsFreeze-fracture Replica ImmunolabelingGlutamate ReceptorsMouse AmygdalaSynaptic StructureProtein DistributionCryo-protectionCopper CarriersVibro-slicerCorrelated Structural And Functional Analysis

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