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Neuroscience

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A Method to Target and Isolate Airway-innervating Sensory Neurons in Mice
 

A Method to Target and Isolate Airway-innervating Sensory Neurons in Mice

Article doi: 10.3791/53917
April 19th, 2016

Summary April 19th, 2016

Organ specific sensory neurons are difficult to identify. Fast Blue tracing is used to identify nodose neurons innervating the airways for cell sorting. Sorted nodose neurons are used to extract high quality ribonucleic acid (RNA) for sequencing. Using this protocol, gene expression of airway specific neurons is determined.

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