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Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
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Developmental Biology
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JoVE Journal Developmental Biology
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

DOI:
10.3791/53974-v

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06:02 min

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June 10, 2016

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, , , , ,
1Department of Clinical Laboratory,The University of Tokyo Hospital, 2Department of Respiratory Medicine,The University of Tokyo Hospital

Chapters

  • 00:05Title
  • 01:01Lung Epithelial Cell Preparation and Culture
  • 01:55Epithelial-mesenchymal Transition (EMT) and Gel Contraction Assay
  • 03:41Results: Representative EMT Confirmation
  • 05:07Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.

Tags

In Vitro AssayMesenchymal CellsEpithelial-mesenchymal Transition (EMT)ContractilityInflammatory CytokinesIdiopathic Pulmonary FibrosisAirway RemodelingA-549 CellsTGF-beta1TNF-alphaGel Medium3D Culture

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