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In - vivo - Biosensor - Spuren Non-apoptotischen Caspase - Aktivität in Drosophila

Article DOI: 10.3791/53992 • 13:21 min
November 27th, 2016 •
1, 1, 2, 1
1W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, 2School of Life Sciences, Chinese University of Hong Kong

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November 27th, 2016

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Um gesunde Zellen in ganzen Tieren erkennen , die geringe Mengen an Caspase - Aktivität enthalten, die hochempfindliche Biosensor bezeichnet CaspaseTracker wurde für Drosophila erzeugt. Caspase-abhängigen Biosensor-Aktivität wird in langlebigen gesunden Zellen in den inneren Organen der erwachsenen Tiere aufgezogen unter optimierten Bedingungen in Abwesenheit von Tod Stimuli detektiert.

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