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Journal / Bioengineering / Circulating MicroRNA Quantification Using…
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JoVE Journal Bioengineering
Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Article DOI: 10.3791/54102-v • 07:37 min • June 26th, 2016
June 26th, 2016 •
1, 2, 2, 2, 3, 4
1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara

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Summary
Automatic Translation

A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.

Tags

Circulating MicroRNA Quantification DNA-binding Dye Chemistry Droplet Digital PCR Biomarker Field Diagnostic And Prognostic Biomarkers High Sensitivity Cancer Diagnostics Early-stage Cancer Detection Neurological Disorders Cardiac Disorders Droplet Manipulation Plasma Or Serum MicroRNAs Isolation Reverse Transcription EvaGreen Master Mix CDNA Preparation DdPCR Mix Working Solution
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