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Journal / Developmental Biology / Isolierung von murinen embryonalen Hemogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolierung von murinen embryonalen Hemogenic Endothelial Cells
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Isolierung von murinen embryonalen Hemogenic Endothelial Cells

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Hämatopoetische Stamm- und Vorläuferzellen (HSPC) leiten sich von spezialisierten (hemogenic) Endothelzellen während der Entwicklung, noch wenig über das Verfahren bekannt, von denen einige Endothelzellen angeben zu werden Blut bilden. Wir zeigen eine Durchflusszytometrie basierende Methode ermöglicht die gleichzeitige Isolierung von hemogenic Endothelzellen und HSPC aus murinen embryonalen Geweben.

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Entwicklungsbiologie Heft 112 hemogenic Endothels definitive Hämatopoese Gefäßentwicklung Maus-Embryonen FACS hämatopoetische Stamm- und Vorläuferzellen
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