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Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi
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Immunology and Infection
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JoVE Journal Immunology and Infection
Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

DOI:
10.3791/54162-v

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12:33 min

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July 06, 2016

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, , ,
1Department of Microbiology and Immunology,Midwestern University

Chapters

  • 00:05Title
  • 01:16HSV Strains and Handling
  • 04:59Polymicrobial Biofilm Assay
  • 07:27Imaging Studies
  • 09:56Results: Microbial Adherence to HSV Infected Cells
  • 11:13Conclusion

Summary

Automatic Translation

Automatic Translation

A method is described herein for the determination of inter-Kingdom association and competition (bacterial and fungal) for adherence to virus-infected HeLa cell monolayers. This protocol can be extended to multiple combinations of prokaryotes, eukaryotes, and viruses.

Tags

Keywords: BiofilmViral InfectionOpportunistic PathogensStaphylococcus AureusCandida AlbicansHSVCMVAdenovirusHeLa CellsC. AlbicansGerm TubeYeast FormAdherenceAttachmentMicrobiomeMultiplicity Of InfectionReporter VirusParaformaldehyde GlutaraldehydeDMEMTrypsinHBSSOD 600

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