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移植到小鼠卵巢脂肪垫

JoVE Journal
Medicine

This content is Free Access.

JoVE Journal Medicine

Transplantation Into the Mouse Ovarian Fat Pad

Please note that all translations are automatically generated. Click here for the English version.

移植到小鼠卵巢脂肪垫

DOI:

10.3791/54444-v

•

09:25 min

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September 07, 2016

•

Andrea Flesken-Nikitin, Blaine A. Harlan, Alexander Yu. Nikitin

¹Department of Biomedical Sciences,Cornell University

Chapters

00:05Title
01:11Isolation and Culture of Primary Tubal Epithelium Cells
02:26Preparation of Uterine Tube and Ovarian Fat Pad Transplantation
05:35Graft Collection and Preservation
06:36Results: Transplantation into the Mouse Ovarian Fat Pad
08:15Conclusion

Summary

Automatic Translation

我们描述适用于女性生殖道的正常和转化上皮研究卵巢脂肪垫移植测定。鼠标脂肪垫允许大型组织碎片移植，是手术和成像方便，并为苗勒起源组织最有利的本地环境。

Tags

Transplantation Ovarian Fat Pad Female Reproductive Tract Epithelial Cells Uterine Tube Primary Tubal Epithelium TE Cells Dissection Orthotopic Transplantation Beta-actin EGFP Beta-actin DS Red PBS Microsurgery Syngeneic Mouse

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