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Enumeration of Neural Stem Cells Using Clonal Assays
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Enumeration of Neural Stem Cells Using Clonal Assays

Enumeration of Neural Stem Cells Using Clonal Assays

DOI:
10.3791/54456-v

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10:32 min

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October 04, 2016

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, , , , , , ,
1Neural Stem Cell Laboratory, Institute of Medical Biology,Agency for Science, Technology and Research (A*STAR)

Chapters

  • 00:05Title
  • 00:50Culture of Clonal Sample Neurospheres
  • 02:07Preparation of Neurosphere-conditioned Medium
  • 04:20Transfer of Sample Neurospheres to the 50-well-chambered Coverslip
  • 06:25Differentiation of Sample Neurospheres
  • 07:18Fixing the Differentiated Neurospheres
  • 08:16Results: Differentiation of Clonal Neurospheres and Enumeration of NSC Frequency
  • 09:28Conclusion

Summary

Automatic Translation

Automatic Translation

Neural stem cells (NSCs) refer to cells which can self-renew and differentiate into the three neural lineages. Here, we describe a protocol to determine NSC frequency in a given cell population using neurosphere formation and differentiation under clonal conditions.

Tags

Keywords: Neural Stem CellsClonal AssaysNeurosphere FormationNeural Stem Cell MarkersNeural ProgenitorsE14.5 MouseNSC Growth MediumTrypan-blue StainingHemocytometerPoly-L-lysineLamininNSC Differentiation Medium

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