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Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
DOI:

10:21 min

November 16, 2016

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Chapters

  • 00:05Title
  • 01:03Outer Membrane Vesicle Purification
  • 03:45Outer Membrane Vesicle Characterization
  • 06:05Verification of Enzyme Packaging
  • 06:53Results: Protein Packaging in Outer Membrane Vesicles
  • 09:03Conclusion

Summary

Automatic Translation

A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

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