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Immunology and Infection

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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Article DOI: 10.3791/54543-v • 10:16 min • September 15th, 2016
September 15th, 2016 •
1, 1, 2, 1, 3, 4, 1, 1
1Multi-Organ Transplant Program, University Health Network, 2Institute of Medical Science, University of Toronto, 3Divison of Neurosurgery, University Health Network, 4Division of Cardiac Surgery, University Health Network

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Summary
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We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.

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Two-color Antigen Microarrays Simultaneous Detection IgG Autoantibodies IgM Autoantibodies Screening Multiplex Fashion Autoimmunity Disease States Advantages Microliters Of Serum Micrograms Of Antigen Generation Diluting Antigens PBS Final Concentration Micro Arrayer Configuration Unique Antigens Duplicate Positive Controls Negative Control Source Plate Foil Freeze Plate Print Arrays Cleaning Solid Microarray Pins Sonicating Bath Deionized Water
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