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Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window
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Cancer Research
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JoVE Journal Cancer Research
Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

DOI:
10.3791/54603-v

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07:19 min

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October 06, 2016

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, , , , ,
1Department of Developmental and Molecular Biology,Albert Einstein College of Medicine, 2Department of Obstetrics/Gynecology and Woman’s Health,Albert Einstein College of Medicine, 3Department of Anatomy & Structural Biology,Albert Einstein College of Medicine, 4Gruss-Lipper Biophotonics Center Integrated Imaging Program,Albert Einstein College of Medicine, 5Medical Research Council Centre for Reproductive Health, Queen’s Medical Research Institute,University of Edinburgh

Chapters

  • 00:05Title
  • 00:44Lung Lobe Exposure
  • 03:25Intravital Imaging
  • 04:47Results: Representative Intravital Vacuum Stabilized Imaging
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of multiphoton microscopy to perform long-term high-resolution, single cell imaging of the intact lung in real time using a vacuum stabilized imaging window.

Tags

Keywords: Long-term ImagingHigh-resolution ImagingIntravital MicroscopyLungVacuum Stabilized Imaging WindowCancer ResearchSingle-cell ResolutionTracheal CatheterDepilatory CreamIntubationVentilatorRib Cage RemovalVacuum WindowPulse OximeterEnvironmental Chamber

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