This protocol describes the use of multiphoton microscopy to perform long-term high-resolution, single cell imaging of the intact lung in real time using a vacuum stabilized imaging window.
Rodriguez-Tirado, C., Kitamura, T., Kato, Y., Pollard, J. W., Condeelis, J. S., Entenberg, D. Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window. J. Vis. Exp. (116), e54603, doi:10.3791/54603 (2016).