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JoVE Journal
Neuroscience

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Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
 

Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

Article doi: 10.3791/54880
December 31st, 2016

Summary December 31st, 2016

This protocol presents a method for the morphological recovery of neurons patched during electrophysiological recordings using biocytin filling and subsequent immunohistochemical postprocessing. We show that thick biocytin-filled sections that were stained and coverslipped can be restained with a second primary antibody days or months later.

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