Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Snezana Vujicic; Lanfei Feng; Angelika Antoni; Joyce Rauch; Jerrold S. Levine

doi:10.3791/54980

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Identificación de eventos de señalización intracelular inducida en células viables por la interacción con las células vecinas sometidos a la célula apoptótica de la muerte

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

JoVE Journal
Biology

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JoVE Journal Biology

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Identificación de eventos de señalización intracelular inducida en células viables por la interacción con las células vecinas sometidos a la célula apoptótica de la muerte

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8,541 Views

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09:18 min

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December 27, 2016

DOI:

10.3791/54980-v

DOI:

10.3791/54980-v

•

09:18 min

December 27, 2016

•

3 Views

Snezana Vujicic*^1,2, Lanfei Feng*^1,2, Angelika Antoni³, Joyce Rauch⁴, Jerrold S. Levine^1,2,5

¹Section of Nephrology, Department of Medicine,University of Illinois at Chicago, ²Section of Nephrology, Department of Medicine,Jesse Brown Veterans Affairs Medical Center, ³Department of Biology,Kutztown University of Pennsylvania, ⁴Division of Rheumatology, Department of Medicine,Research Institute of the McGill University Health Centre, ⁵Department of Microbiology & Immunology,University of Illinois at Chicago

Transcript

Summary

Here, we present a protocol for the determination of intracellular signaling events induced in viable cells by physical interaction with adjacent dead or dying cells. The protocol focuses on signaling events induced by receptor-mediated recognition of the dead cells, as opposed to their phagocytic uptake or release of soluble mediators.