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JoVE Journal
Developmental Biology

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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
 

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

Article DOI: 10.3791/55134
January 13th, 2017

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Summary January 13th, 2017

We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

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