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Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

DOI:
10.3791/55262-v

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08:13 min

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March 04, 2017

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, , , ,
1Analytical Research & Development,Sanofi Pasteur Limited

Chapters

  • 00:05Title
  • 01:11Instrument Start-up
  • 02:08Sample Preparation
  • 03:46Experimental Parameter Setup
  • 05:46Results: Thermal Transitions of Vaccine Antigens
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

Differential scanning calorimetry measures the thermal transition temperature(s) and total heat energy required to denature a protein. Results obtained are used to assess the thermal stability of protein antigens in vaccine formulations.

Tags

Keywords: Differential Scanning CalorimetryThermal StabilityProtein AntigenStructural ConformationMolar Heat CapacityThermal Transition TemperatureEnthalpy Of UnfoldingLot-to-lot ConsistencyBiologicsProtein ConcentrationLowry MethodBuffer Equilibration

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