Journal
/
Neuroscience
/
Measuring and Altering Mating Drive in Male Drosophila melanogaster
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Measuring and Altering Mating Drive in Male Drosophila melanogaster

Measuring and Altering Mating Drive in Male Drosophila melanogaster

DOI:
10.3791/55291-v

•

07:02 min

•

February 15, 2017

•

, , ,
1F.M. Kirby Neurobiology Center,Boston Children’s Hospital, 2Harvard NeuroDiscovery Center,Harvard Medical School, 3Department of Neurobiology,Harvard Medical School

Chapters

  • 00:05Title
  • 00:38Preparing Virgin Females for Behavioral Assays
  • 01:27Food Preparation for 2-D Satiety Assay
  • 02:36Performing and Scoring 2-D Satiety Assays
  • 04:10Using Courtship to Verify Satiety
  • 04:58Recovering Mating Drive after 2-D Satiety Assay
  • 05:29Results: Reversible Decline in Male Mating Drive over Time
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.

Tags

Keywords: Drosophila MelanogasterMating DriveMotivationHigh-throughput Assay2-D Satiety AssayVirgin FemalesGenetic ToolsHeat ShockFood PreparationIncubatorHumidity Control

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55291?article_type=v

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae
check_url/55291?article_type=v

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster
check_url/55291?article_type=v

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster
check_url/55291?article_type=v

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster
check_url/55291?article_type=v

Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized Drosophila
check_url/55291?article_type=v

Conditions Affecting Social Space in Drosophila melanogaster
check_url/55291?article_type=v

Foraging Path-length Protocol for Drosophila melanogaster Larvae
check_url/55291?article_type=v

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster
check_url/55291?article_type=v

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains
check_url/55291?article_type=v

Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster

Read Article