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Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
 

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

Article doi: 10.3791/55514
April 9th, 2017

Summary April 9th, 2017

This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.

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