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La inducción de la mesenquimales-epitelial Transitions en células de sarcoma
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La inducción de la mesenquimales-epitelial Transitions en células de sarcoma

Article DOI: 10.3791/55520-v • 11:42 min • April 7th, 2017
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

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Presentamos aquí un método de cultivo celular para inducir transiciones mesenquimales-epitelial (MET) en células de sarcoma en base a la expresión ectópica combinado de miembros de la familia microRNA-200 y Grainyhead-como 2 (GRHL2). Este método es adecuado para una mejor comprensión del impacto biológico de la plasticidad fenotípica de la agresividad del cáncer y los tratamientos.

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Developmental Biology Número 122 transiciones mesenquimales-epitelial sarcoma transfección microRNA-200 familia GRHL2 cultivo celular la plasticidad epitelial
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