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Developmental Biology
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5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平<em>体外</em
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Developmental Biology
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JoVE Journal
Developmental Biology
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平<em>体外</em
DOI:
10.3791/55565-v
•
10:07 min
•
May 17, 2017
•
Zhaofeng Jia
2
,
Yujie Liang
,
Bin Ma
5
,
Xiao Xu
6
,
Jianyi Xiong
,
Li Duan
,
Daping Wang
2
1
Guangzhou Medical University
,
2
Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics
,
Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University)
,
3
Department of Chemistry
,
The Chinese University of Hong Kong
,
4
School of Biomedical Engineering
,
Shanghai Jiao Tong University
,
5
Renji Hospital Clinical Stem Cell Research Center
,
Shanghai Jiao Tong University School of Medicine
,
6
Shantou University Medical College
Chapters
00:05
Title
01:52
Human Articular Cartilage Tissue Collection and Chondrocyte Culture
03:33
Genomic DNA Extraction
05:47
Dot Blot nalysis
07:55
Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
08:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一种基于5-甲基胞嘧啶(5-mC)斑点印迹定量DNA甲基化的方法。我们在软骨细胞去分化中测定了5-mC的水平。这种简单的技术可用于快速测定ACI治疗中的软骨细胞表型。
Tags
5-mC Dot Blot
DNA Methylation
Chondrocyte Dedifferentiation
Autologous Chondrocyte Implantation
Cartilage Defects
Osteoarthritis
Genomic DNA
Nylon Membrane
Collagenase II
Cell Culture
Cell Passage
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