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5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平<em>体外</em
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
DOI:

10:07 min

May 17, 2017

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Chapters

  • 00:05Title
  • 01:52Human Articular Cartilage Tissue Collection and Chondrocyte Culture
  • 03:33Genomic DNA Extraction
  • 05:47Dot Blot nalysis
  • 07:55Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
  • 08:45Conclusion

Summary

Automatic Translation

我们提出了一种基于5-甲基胞嘧啶(5-mC)斑点印迹定量DNA甲基化的方法。我们在软骨细胞去分化中测定了5-mC的水平。这种简单的技术可用于快速测定ACI治疗中的软骨细胞表型。

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