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Developmental Biology

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In Ovo Electroporation in the Chicken Auditory Brainstem

In Ovo Electroporation in the Chicken Auditory Brainstem

Article DOI: 10.3791/55628 10:14 min June 9th, 2017
June 9th, 2017



Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development.



In Ovo Electroporation Gene Expression Chicken Auditory Brainstem Auditory Neuron Development Pathophysiological Phenomena Gain Or Loss Of Phenotype Plasmid Solutions Fast Green Solution DNA Mixture Simultaneous Electroporation Pipettes Micropipette Puller 28-gauge Syringe Needle Microscope Picospritzer Egg Storage Disinfection
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