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Vurdering af Sarcoplasmic Reticulum Calcium Reserve og intracellulære diastolisk Calcium fjernelse i isolerede ventrikulær Cardiomyocytes
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JoVE Journal Medicine
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

Vurdering af Sarcoplasmic Reticulum Calcium Reserve og intracellulære diastolisk Calcium fjernelse i isolerede ventrikulær Cardiomyocytes

DOI:
10.3791/55797-v

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11:00 min

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September 18, 2017

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, , , , , ,
1Department of Cardiology, Sir Run Run Shaw Hospital,Zhejiang University School of Medicine, 2Experimental Research Center,China Academy of Chinese Medical Sciences, 3Department of Cardiology,The Third Hospital of Hangzhou City

Chapters

  • 00:05Title
  • 00:44Isolation of Left Ventricular Cardiomyocytes
  • 04:15Set Up of Perfusion System
  • 04:59Measurement of Intracellular Ca2+ Transient and Sarcomere Shortening
  • 06:51Assessment of SR Ca2+ Reserve and Diastolic Ca2+ Removal Function
  • 09:10Results: Assessment of SR Calcium Reserve and Calcium Removal Function
  • 10:17Conclusion

Summary

Automatic Translation

Automatic Translation

Intracellulære calcium genanvendelse spiller en afgørende rolle i reguleringen af systolisk og diastolisk funktion i cardiomyocytes. Her, beskriver vi en protokol for at vurdere sarcoplasmic reticulum Ca2 + reserve og diastolisk calcium fjernelse funktion i cardiomyocytes af en calcium imaging system.

Tags

Sarcoplasmic ReticulumCalcium ReserveIntracellular Diastolic Calcium RemovalIsolated Ventricular CardiomyocytesLangendorff PerfusionNT SolutionCalcium-free Tyrode SolutionCollagenase AKB SolutionCell IsolationCell FiltrationCentrifugation

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