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Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
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Neuroscience
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JoVE Journal Neuroscience
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

DOI:
10.3791/55862-v

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07:30 min

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September 04, 2017

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, ,
1National Institute of Neurological Disorders and Stroke, 2College of Pharmacy,Chung-ang University

Chapters

  • 00:05Title
  • 00:40Culture Stimulation
  • 01:35Fixation and Staining
  • 03:42Epoxy Embedding
  • 05:43Results: Images of Presynaptic Terminal in Stimulated Neurons with High Potassium
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

Synaptic vesicle endocytosis is detected by light microscopy of pHluorin fused with synaptic vesicle protein and by electron microscopy of vesicle uptake.

Tags

Synaptic Vesicle EndocytosisHippocampal NeuronsPoly-d-lysineHigh Potassium StimulationGlutaraldehydeSodium Cacodylate BufferDAB SolutionOsmium TetroxideSodium Acetate BufferUranyl AcetateDehydrationElectron Microscopy

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