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Genetics
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染色质免疫沉淀(ChIP)在小鼠T细胞系
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
Please note that all translations are automatically generated.
Click here for the English version.
染色质免疫沉淀(ChIP)在小鼠T细胞系
DOI:
10.3791/55907-v
•
11:39 min
•
June 17, 2017
•
Benedetto Daniele Giaimo
,
Francesca Ferrante
,
Tilman Borggrefe
1
Institute of Biochemistry
,
University of Giessen
Chapters
00:05
Title
01:01
Crosslinking the Cells, Cell Lysis, and Chromatin Shearing
03:31
Determination of Shearing Efficiency
05:55
Immunoprecipitation
08:09
DNA Purification
09:09
Results: Shearing Efficiency and Distribution of Chromatin Marks in Mouse T-cell Lines
10:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作描述了使用成熟小鼠T细胞系的染色质免疫沉淀(ChIP)的方案。该方案适用于研究特定启动子位点或全基因组特异性组蛋白标记的分布。
Tags
Chromatin Immunoprecipitation
ChIP
Mouse T-cell Lines
Histone Marks
Chromatin Biology
Chromatin Shearing
Formaldehyde Crosslinking
Glycine Quenching
Sonication
Focused Ultrasonicator
Lysis Buffer
Cell Pellet
Supernatant
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