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Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation
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Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation

Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation

DOI:
10.3791/55917-v

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08:30 min

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August 14, 2017

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, , , ,
1Department of Pathology and Molecular Medicine,Queen’s University, 2Department of Medicine,Queen’s University

Chapters

  • 00:05Title
  • 01:02Endothelial Cell Flow Chamber Seeding
  • 02:26Flow Apparatus Assembly and Miroscope Setup
  • 04:15von Willebrand Factor (VWF)-Platelet String Formation and Quantification
  • 07:05Results: Histones Mediate VWF-Platelet String Formation
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

In this paper, we describe a method to assess endothelial von Willebrand factor release and the subsequent platelet capture under fluid shear stress in response to inflammatory stimuli using an in vitro flow chamber system.

Tags

Keywords: Von Willebrand FactorVWF-platelet String FormationFlow Chamber ModelCollagenEndothelial CellsPlateletsSecretagoguesIn VitroMicroscopySyringe Pump

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