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Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
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Chapters
- 00:04Title
- 00:42Stereotaxic Injection of Tracer
- 02:54Perfusion and Sectioning
- 04:15Double Fluorescent Staining for BDA and Basic Neural Elements in Cerebral Cortex
- 05:07Results: Injection Site and Typical Neural Labeling with High Molecular Weight BDA in the Somatosensory Cortex
- 06:04Conclusion
Here, we present a refined protocol to effectively reveal biotinylated dextran amine (BDA) labeling with a fluorescent staining method through a reciprocal neural pathway. It is suitable for analyzing the fine structure of BDA labeling and distinguishing it from other neural elements under a confocal laser scanning microscope.
