Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila

The overall goal of this technique is to visualize single cell morphologies in complex anatomical tissues, thus simplifying the study of cell cell interaction in Drosophila. This method can help to answer key questions regarding complex morphologies and cell cell interactions using the fruit fly. The main advantage of this technique is that it can be adapted to the study of single cell morphologies in any tissue and at any developmental stage.

Demonstrating the procedure will be Anja Kieser, a technician in our laboratory. The MCFO technique modifies the standard FlpOut technique as follows. A heat shock FLP recombinase and different reporters remain under UAS control, however, each reporter has a common backbone of a myristoylated superfolder GFP, in which copies of an epitope tag have been inserted.