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Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila
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JoVE Journal Behavior
Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila

Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila

DOI:
10.3791/56177-v

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08:30 min

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October 20, 2017

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, , ,
1Gene Center and Department of Biochemistry,Ludwig-Maximilians-University Munich, 2Janelia Farm Research Campus,Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 00:41Preparing Flies for MultiColor FlpOut (MCFO) Experiments
  • 02:35Adult Brain Dissection
  • 04:26Adult Brain Staining and Mounting
  • 06:39Results: Spaghetti Monster GFP (smGFP) Marker Combinations Labeling Antennal Lobe Cells
  • 07:48Conclusion

Summary

Automatic Translation

Automatic Translation

Cells display different morphologies and establish a variety of interactions with their neighbors. This protocol describes how to reveal the morphology of single cells and to investigate cell-cell interaction by using the well-established Gal4/UAS expression system.

Tags

Keywords: MultiColor FlpOut TechniqueSingle Cell MorphologiesCell InteractionsDrosophilaGlial CellsHeat ShockFLP RecombinaseReportersSpaghetti Monster GFPsEpitope TagsGAL4 DriverDissectionPBSEthanolS2 Cell Culture Medium

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