Journal
/
Chemistry
/
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

DOI:
10.3791/56253-v

•

08:05 min

•

September 29, 2017

•

, , ,
1The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry,Tsinghua University, 2Institute of Chemical Materials,China Academy of Engineering Physics

Chapters

  • 00:05Title
  • 00:59Synthesis of DF PEG for Hydrogels
  • 03:00Preparing Chitosan-based Hydrogels with Encapsulated Cells
  • 05:15Cell Viability Analysis
  • 06:08Results: Affect of Injection on Viability of Hydrogel-encapsulated L929 Mouse Fibroblasts
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a facile preparation of chitosan-based injectable hydrogels using dynamic imine chemistry. Methods to adjust the hydrogel’s mechanical strength and its application in 3D cell culture are presented.

Tags

ChitosanInjectable Hydrogel3D Cell CultureSelf-healing HydrogelDF PEGBenzaldehyde-terminated DF PEGCell EncapsulationCell Culture MediaCell Therapy Carrier

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography

Preparation and Use of Carbonyl-decorated Carbenes in the Activation of White Phosphorus

Assessment of Boron Doped Diamond Electrode Quality and Application to In Situ Modification of Local pH by Water Electrolysis

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles

Photogeneration of N-Heterocyclic Carbenes: Application in Photoinduced Ring-Opening Metathesis Polymerization

Synthesis of Soft Polysiloxane-urea Elastomers for Intraocular Lens Application

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

Read Article