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Um método geral para detectar a formação de Nitrosamide no metabolismo In Vitro de nitrosaminas por citocromo P450s

Article DOI: 10.3791/56312-v • 07:38 min • September 25th, 2017
September 25th, 2017 •
1,2, 2, 2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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Α-hidroxilação de nitrosaminas cancerígenas pelo citocromo P450s é a aceita via metabólica que produz o DNA de danos intermediários, que causam mutações. No entanto, novos dados indicam mais pode ocorrer oxidação para nitrosamides. Descrevemos um general método para a detecção de nitrosamides produzidos a partir de em vitro citocromo P450-catalisada metabolismo de nitrosaminas.

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Biologia celular edição 127 nitrosamina nitrosamide metabolismo do citocromo P450 oxidação processive carcinogênese espectrometria de massa de alta resolução
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