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Cancer Research
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Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

DOI:
10.3791/56327-v

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06:44 min

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September 08, 2017

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, , , , ,
1Department of Pathology, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai, 2Department of Oncological Sciences, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:05Title
  • 00:52Generation of Docetaxel-resistant Prostate Cancer Cells
  • 03:33Functional Characterization of Acquired Docetaxel Resistance by Colony Formation Assay
  • 05:10Results: Generation of Docetaxel-resistant Prostate Cancer Cell Model Systems and Functional Characterization of Docetaxel Resistance
  • 06:14Conclusion

Summary

Automatic Translation

Automatic Translation

Resistance to cancer therapies contributes to disease progression and death. Determining the mechanistic underpinnings of resistance is crucial for improving therapeutic response. This manuscript details the protocol to generate taxane-resistant cell models of prostate cancer (PC) to help dissecting the pathways involved in progression to Docetaxel resistance in PC patients.

Tags

Docetaxel-resistant Prostate Cancer Cell ModelsCancer Cell ResistanceAnti-mitotic Therapy ResistanceDrug Response ModelsCell Line DevelopmentDU145 Cells22Rv1 CellsDose EscalationCell CultureTrypsinizationCell PassagingPooled Resistant Cells

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