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Immunology and Infection

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病毒 DNA 的纯化及相关病毒和细胞蛋白的鉴定

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Immunology and Infection

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JoVE Journal Immunology and Infection

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Please note that all translations are automatically generated. Click here for the English version.

病毒 DNA 的纯化及相关病毒和细胞蛋白的鉴定

DOI:

10.3791/56374-v

•

08:26 min

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August 31, 2017

•

Jill A. Dembowski, Neal A. Deluca

¹Department of Microbiology and Molecular Genetics,University of Pittsburgh School of Medicine

Chapters

00:05Title
00:37Viral Infection and EdC Labeling
01:57Purification of Viral DNA and Associated Proteins
06:21Results: Protein Purification and Proteomics Data
07:36Conclusion

Summary

Automatic Translation

该协议的目的是专门标记和有选择地分离病毒 DNA 从受感染的细胞, 以表征病毒基因组相关的蛋白质。

Tags

Keywords: Viral DNA Purification Herpes Simplex Virus Type 1 Proteomic Analysis Cellular Factors Viral Genome MRC-5 Cells HSV-1 Infection Viral DNA Replication EdC Labeling Nuclei Extraction Nuclear Pellet Click Reaction Cellular Proteins

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